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primary human alveolar type ii epithelial cells  (Procell Inc)

 
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    Procell Inc primary human alveolar type ii epithelial cells
    Primary Human Alveolar Type Ii Epithelial Cells, supplied by Procell Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 86 stars, based on 1 article reviews
    primary human alveolar type ii epithelial cells - by Bioz Stars, 2026-06
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    TGF‐β1 promotes EMT process and decreases miR‐29b‐2‐5p and miR‐34c‐3p levels. A, Western blot analysis of E‐cadherin, Vimentin, Fibronectin and α‐SMA in A549 cells and BEAS‐2B cells treated with 0, 1, 2 and 5 ng/mL TGF‐β1 for 48 h. B, Western blot analysis of E‐cadherin, Vimentin, Fibronectin and α‐SMA in human primary type II AECs treated with 0 or 5 ng/mL TGF‐β1 for 48 h. C, Wound healing assays were performed to measure the migration ability of A549 cells treated with 0, 1, 2 and 5 ng/mL TGF‐β1 for 48 h. D, Immunofluorescence staining of Vimentin and E‐cadherin in A549 cells for the control and TGF‐β1 (0 or 5 ng/mL) treatment groups. Green represents Vimentin staining; red represents E‐cadherin staining; and blue represents nuclear DNA staining by DAPI. The scale bar is 50 μm. E, qRT‐PCR detection of lncRNA‐ATB expression in 0, 1, 2 and 5 ng/mL TGF‐β1 treated A549 and BEAS‐2B cells for 48 h (mean ± SD, n = 3), * P < .05 difference from untreated cells. F, qRT‐PCR analysis of miR‐29b‐2‐5p and miR‐34c‐3p expression in 0, 1, 2 and 5 ng/mL TGF‐β1 treated A549 and BEAS‐2B cells for 48 h (mean ± SD, n = 3), * P < .05 difference from untreated cells. G, qRT‐PCR detection of lncRNA‐ATB, miR‐29b‐2‐5p and miR‐34c‐3p expression in human primary type II AECs after 0 or 5 ng/mL TGF‐β1 treatment (mean ± SD, n = 3), * P < .05

    Journal: Journal of Cellular and Molecular Medicine

    Article Title: LncRNA‐ATB regulates epithelial‐mesenchymal transition progression in pulmonary fibrosis via sponging miR‐29b‐2‐5p and miR‐34c‐3p

    doi: 10.1111/jcmm.16758

    Figure Lengend Snippet: TGF‐β1 promotes EMT process and decreases miR‐29b‐2‐5p and miR‐34c‐3p levels. A, Western blot analysis of E‐cadherin, Vimentin, Fibronectin and α‐SMA in A549 cells and BEAS‐2B cells treated with 0, 1, 2 and 5 ng/mL TGF‐β1 for 48 h. B, Western blot analysis of E‐cadherin, Vimentin, Fibronectin and α‐SMA in human primary type II AECs treated with 0 or 5 ng/mL TGF‐β1 for 48 h. C, Wound healing assays were performed to measure the migration ability of A549 cells treated with 0, 1, 2 and 5 ng/mL TGF‐β1 for 48 h. D, Immunofluorescence staining of Vimentin and E‐cadherin in A549 cells for the control and TGF‐β1 (0 or 5 ng/mL) treatment groups. Green represents Vimentin staining; red represents E‐cadherin staining; and blue represents nuclear DNA staining by DAPI. The scale bar is 50 μm. E, qRT‐PCR detection of lncRNA‐ATB expression in 0, 1, 2 and 5 ng/mL TGF‐β1 treated A549 and BEAS‐2B cells for 48 h (mean ± SD, n = 3), * P < .05 difference from untreated cells. F, qRT‐PCR analysis of miR‐29b‐2‐5p and miR‐34c‐3p expression in 0, 1, 2 and 5 ng/mL TGF‐β1 treated A549 and BEAS‐2B cells for 48 h (mean ± SD, n = 3), * P < .05 difference from untreated cells. G, qRT‐PCR detection of lncRNA‐ATB, miR‐29b‐2‐5p and miR‐34c‐3p expression in human primary type II AECs after 0 or 5 ng/mL TGF‐β1 treatment (mean ± SD, n = 3), * P < .05

    Article Snippet: Human primary type II alveolar epithelial cells (AECs) were obtained from Procell Biotechnology and cultured in human type II alveolar epithelial cell medium.

    Techniques: Western Blot, Migration, Immunofluorescence, Staining, Control, Quantitative RT-PCR, Expressing

    Levels of HMGB-1 in the culture supernatant of human pulmonary alveolar epithelial cells (HPAEpiCs) after stimulation with 20 μM synthetic Hz and treatment with different concentrations (1, 5, and 10 μg/ml) of anti-HMGB-1 monoclonal antibody for different times (0, 4, 12, 24, and 48 h). The levels of HMGB-1 were measured by ELISA. The data are expressed as the mean ± SEM of 3 independent experiments

    Journal: Iranian Journal of Parasitology

    Article Title: Protective Effect of an Anti-HMGB-1 Neutralizing Antibody on Hemozoin-Induced Alveolar Epithelial Cell in a Model of Malaria Associated ALI/ARDS

    doi: 10.18502/ijpa.v16i3.7089

    Figure Lengend Snippet: Levels of HMGB-1 in the culture supernatant of human pulmonary alveolar epithelial cells (HPAEpiCs) after stimulation with 20 μM synthetic Hz and treatment with different concentrations (1, 5, and 10 μg/ml) of anti-HMGB-1 monoclonal antibody for different times (0, 4, 12, 24, and 48 h). The levels of HMGB-1 were measured by ELISA. The data are expressed as the mean ± SEM of 3 independent experiments

    Article Snippet: Primary human pulmonary alveolar epithelial cells (HPAEpiCs, type II alveolar epithelial cells) were obtained from ScienCell Research Laboratories (Catalog no. 3200, ScienCell Research Laboratories, USA).

    Techniques: Enzyme-linked Immunosorbent Assay

    The levels of TNF-α and IFN-γ in the culture supernatant of human pulmonary alveolar epithelial cells (HPAEpiCs) after stimulation with 20 μM synthetic Hz and treatment with different concentrations (1, 5, and 10 μg/ml) of anti-HMGB-1 monoclonal antibody for different times (0, 4, 12, 24, and 48 h). (a) The level of TNF-α in the culture supernatant. (b) The level of IFN-γ in the culture supernatant. The data are expressed as the mean ± SEM of 3 independent experiments

    Journal: Iranian Journal of Parasitology

    Article Title: Protective Effect of an Anti-HMGB-1 Neutralizing Antibody on Hemozoin-Induced Alveolar Epithelial Cell in a Model of Malaria Associated ALI/ARDS

    doi: 10.18502/ijpa.v16i3.7089

    Figure Lengend Snippet: The levels of TNF-α and IFN-γ in the culture supernatant of human pulmonary alveolar epithelial cells (HPAEpiCs) after stimulation with 20 μM synthetic Hz and treatment with different concentrations (1, 5, and 10 μg/ml) of anti-HMGB-1 monoclonal antibody for different times (0, 4, 12, 24, and 48 h). (a) The level of TNF-α in the culture supernatant. (b) The level of IFN-γ in the culture supernatant. The data are expressed as the mean ± SEM of 3 independent experiments

    Article Snippet: Primary human pulmonary alveolar epithelial cells (HPAEpiCs, type II alveolar epithelial cells) were obtained from ScienCell Research Laboratories (Catalog no. 3200, ScienCell Research Laboratories, USA).

    Techniques:

    The mRNA expression of RAGE, TLR-2 and TLR-4 in human pulmonary alveolar epithelial cells (HPAEpiCs) after stimulation with 20 μM synthetic Hz and treatment with different concentrations (1, 5, and 10 μg/ml) of anti-HMGB-1 monoclonal antibody for different times (0, 4, 12, 24, and 48 h). (a) The mRNA expression of RAGE. (b) The mRNA expression of TLR-2. (c) The mRNA expression of TLR-4. The data are expressed as the mean ± SEM of 3 independent experiments. * P <0.05

    Journal: Iranian Journal of Parasitology

    Article Title: Protective Effect of an Anti-HMGB-1 Neutralizing Antibody on Hemozoin-Induced Alveolar Epithelial Cell in a Model of Malaria Associated ALI/ARDS

    doi: 10.18502/ijpa.v16i3.7089

    Figure Lengend Snippet: The mRNA expression of RAGE, TLR-2 and TLR-4 in human pulmonary alveolar epithelial cells (HPAEpiCs) after stimulation with 20 μM synthetic Hz and treatment with different concentrations (1, 5, and 10 μg/ml) of anti-HMGB-1 monoclonal antibody for different times (0, 4, 12, 24, and 48 h). (a) The mRNA expression of RAGE. (b) The mRNA expression of TLR-2. (c) The mRNA expression of TLR-4. The data are expressed as the mean ± SEM of 3 independent experiments. * P <0.05

    Article Snippet: Primary human pulmonary alveolar epithelial cells (HPAEpiCs, type II alveolar epithelial cells) were obtained from ScienCell Research Laboratories (Catalog no. 3200, ScienCell Research Laboratories, USA).

    Techniques: Expressing